Five great tricks to buy ELISA kits! Hurry and watch! -Huaqiang Electronic Network
Shanghai Jinma Biotechnology Co., Ltd. offers both domestic and imported sub-package original ELISA kits, all professionally certified. Once you receive the kit, you can immediately perform experiments with confidence, as the quality is reliable, and results are accurate and stable. When choosing an ELISA kit, it's essential to consider several key factors. Kits available in the market include both imported and locally developed options. To select one that meets your detection needs while being cost-effective, look at the specifications provided in the kit instructions. These usually include the minimum detection limit, recovery rate, cross-reactivity rate, standard curve data, and IC50 values. A standard curve should have at least five concentrations, and the number of data points should be sufficient for comparison. Determining the cut-off value is a crucial step during actual testing. This threshold helps identify whether a sample is negative or suspicious. For banned substances, the cut-off value corresponds to the limit of quantitation—this is the lowest level that can be reliably measured using existing instruments. For drugs with maximum residue limits (MRL), the cut-off is calculated by adding 20 samples of MRL concentration and subtracting 1.64 standard deviations from the mean of three measurements. If the result is below the cut-off, it's considered negative; if above, further confirmation is required. The detection limit should also be evaluated by checking the background noise of the kit. This is done by measuring 20 blank samples and adding three standard deviations. If the detection limit exceeds the cut-off, it may indicate high background interference, and the kit might not meet sensitivity requirements. In such cases, contacting the manufacturer or using a different kit is advisable. Method verification is essential when performing ELISA tests. Key aspects include: - Constructing a standard curve with at least five concentrations and repeating measurements to determine the working range and IC50. - Assessing the detection limit of the sample. - Conducting recovery experiments by spiking samples with known concentrations. For banned drugs, a low cross-reaction rate is important. Recovery rates should exceed 40%, and intra-assay and inter-assay coefficients of variation should be within acceptable ranges (≤25% for general samples, ≤30% for banned drugs). Meeting these standards ensures the ELISA method is reliable and suitable for detecting drug residues. Additional tips for testing include: - Using multi-residue kits with low cross-reactivity for better accuracy. - Including at least two standard curves, two negative controls, two positive cut-off controls, and two parallel samples during testing. - Checking the shape of the standard curve (S-shaped), ensuring the IC50 is close to the cut-off, and verifying the recovery rate and parallelism of the samples. All ELISA kits from Shanghai Jinma Biotech come with free testing support. From experimental guidance to hands-on assistance, we provide comprehensive pre-sale, sale, and after-sales services. Both new and returning customers enjoy discounts and dedicated support throughout the process.
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