Correct writing method for ELISA kit experimental records - Database & Sql Blog Articles

ELISA Kit

Experimental data collection and documentation are essential parts of the scientific research process. These records serve as the foundation for future analysis and help ensure the reliability of results. However, certain bad habits can compromise the accuracy, timeliness, and completeness of these records. Here are some common issues to avoid:

1. Not carrying an experimental notebook when conducting experiments. Some people tend to write down observations on scraps of paper or other materials nearby, only to transfer them later. This often leads to loss of details, forgotten information, or misplaced notes. To prevent this, it's important to always carry your notebook and record data immediately during the experiment.

2. Delayed recording of results. Some researchers rely on memory to recall experimental procedures, which can lead to incomplete or inaccurate records. The saying "a poor memory is better than a good one" holds true here. If you don’t document your findings in real time, important details may be lost, especially if changes were made during the experiment that aren't recorded. For example, a researcher once missed a key condition change that led to a successful outcome, causing a delay of six months in repeating the same result.

3. Failing to organize and analyze data promptly. Without timely organization, it’s hard to identify patterns or make informed decisions for future experiments. Rushing into new tests without reviewing previous results can lead to repeated errors, wasted resources, and missed opportunities for deeper insights. Taking time to reflect and analyze after each experiment can save time and improve efficiency in the long run.

4. Not recording dates and times accurately. Time passes quickly, and without proper timestamps, it becomes difficult to trace back the sequence of events. Many researchers only note the day and month, but for long-term storage, including the year is crucial. Also, failing to record exact times can cause inconsistencies in results, especially when analyzing trends over time.

5. Discarding negative results. All data—positive or negative—should be preserved because they can provide valuable insights. A result considered "negative" at first might later prove to be critical in understanding the experiment’s success or failure. It’s important to document everything, as even failures can lead to breakthroughs.

6. Only recording what you expect to see. Experimental records should capture all actual observations, not just those that align with your expectations. Even mistakes and unexpected outcomes can help refine hypotheses and guide future experiments. Keeping a detailed account of every step ensures that the full story of the experiment is available for review and interpretation.

Writing Requirements for Experimental Records

Basic Requirements:

1. All original data must be recorded in the official lab notebook. Pages should be numbered sequentially and not removed or torn out.

2. The first page of the notebook serves as a table of contents and can be filled in at the start or end of an experiment.

3. Each entry should include the date (year, month, day) and time, along with environmental conditions like temperature and humidity.

4. Writing should be clear, using standard scientific terminology, units, and abbreviations. When using an abbreviation for the first time, write out the full name and its Chinese equivalent.

5. If corrections are needed, strike through the original text and sign the correction. Do not erase or use smudged ink.

6. Raw data, figures, charts, and photos should be directly recorded or attached to the notebook to ensure permanent documentation.

7. Experimental records are essential for publishing papers and managing lab files. Students must submit all records to the lab before leaving school.

Content of the Experimental Record:

1. Date and time of the experiment, along with environmental conditions.

2. Experiment title.

3. Purpose of the experiment.

4. Materials used.

5. Reagents: name, batch number, manufacturer, concentration, solvent, and storage conditions.

6. Instruments: name, model, and supplier.

7. Cells or bacteria: name, resuscitation method, cryopreservation, and storage.

8. Animals: strain, source, age, gender, and quantity.

9. Clinical samples: patient information, diagnosis, and other clinical data.

10. Preparation of reagents.

11. Detailed experimental procedure.

12. Results: raw data, observations, and final outcomes.

13. Problems encountered: possible causes and solutions should be documented.

14. Summary: brief conclusion, lessons learned, and recommendations for future work.

Key Points to Note:

1. Never write notes the next day or on loose paper.

2. Ensure authenticity and completeness of records. Always note the date.

3. Original data, including images, must be posted in the notebook on the day of the experiment.

4. Even negative results should be included. Don’t only record what fits your expectations.

5. Regularly organize and analyze data, and report findings to your supervisor.