Five great tricks to buy ELISA kits! Hurry and watch! -Huaqiang Electronic Network
Shanghai Jinma Biotechnology Co., Ltd. offers both domestic and imported sub-package original ELISA kits, all professionally certified. Once you receive the kit, you can start your experiment immediately. The quality is reliable, and the results are accurate and stable. When selecting an ELISA kit, it's important to consider several key factors. Kits come in various types—some imported, others developed locally. Choosing one that fits your detection needs and budget can be challenging. Based on practical testing, I’ve found that the kit instructions usually list critical parameters such as minimum detection limit, recovery rate, cross-reactivity rate, standard curve data, and IC50 values. A standard curve should include at least five concentrations, and the number of measurements should be no less than five. These specifications help in making an informed choice. Determining the cut-off value is a crucial step when using ELISA. By comparing test results with the cut-off, you can classify samples as negative or suspicious. For banned drugs, the cut-off value represents the limit of quantitation—the lowest level detectable by current accepted methods. For drugs with maximum residue limits (MRL), the cut-off is calculated using 20 samples at MRL concentration, taking the mean of three readings and subtracting 1.64 standard deviations. If the result is below the cut-off, it’s considered negative; if equal or above, it’s suspicious and requires further confirmation. It’s also important to check the detection limit for each lab. This is calculated as the average of 20 blank samples plus three standard deviations. If this value exceeds the cut-off, it suggests high background interference and poor sensitivity. In such cases, contacting the manufacturer or replacing the kit is recommended. Method verification is essential before conducting actual tests. Key aspects include: 1. Constructing a standard curve with at least five concentrations and repeated measurements to determine working ranges and IC50. 2. Evaluating the sample detection limit. 3. Conducting recovery experiments. For banned substances, add two levels around the limit of quantitation. For MRLs, add concentrations above and below the limit. Accuracy and precision standards must be met: recovery rates over 40%, intra-assay CV ≤25% (≤30% for banned drugs), and inter-assay CV ≤30% (≤40% for banned drugs). Meeting these ensures the method is valid for drug residue detection. Additional considerations include using multi-residue kits with low cross-reactivity, and including at least two standard curves, two negative controls, two positive cut-off controls, and two parallel samples during testing. After obtaining results, check the standard curve shape, IC50 proximity to the cut-off, and sample recovery to ensure reliability. All ELISA kits from Shanghai Jinma Biotech are available for free testing. We provide full technical support, free experimental guidance, and consistent service from pre-sale to after-sales. Both new and returning customers enjoy discounts and dedicated assistance.
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